
This image is of the post-PCR cleanup of the single colonies from the culture dependent project that will be sent for sequencing at UPenn. Lanes 9, 10, and 13 are faint because dye "floating" was experienced again, nonetheless, these bands are still visible on the gel. Not all samples were loaded on the gel, but this gel confirms that the procedure work, so it may not be worthwhile to run a new gel.