Lasergene Manual

So, in anticipation of successful results from sequencing, here is the link to the Lasergene manual. The stuff about running BLAST searches starts on page 8.

PCR Cleanup, 11.6

PCR cleanup appears to have been successful. No primers remain, and there are products from all four cleanups. See the note in the photo regarding band intensity. Ready for ligations, once concentrations and ligation procedure has been finalized.

Gel of PCR products from this morning. All but one of the elution 1 samples amplified. The scan isn't very good, but looking at the hard copy it is possible to see that all elution 2 samples amplified. We plan to pool elution 1 and all elution 2 samples and do PCR clean-up.

E & F

PCR Cleanup, gel image

Image from gels with PCR cleanup products. These products represent S2, S7, A2 and A7, all from elution two. Based on the ladder, the clean product appears to be around 500 bp in length.

Hey everyone,

Ok, so here's is the gel from our second try at PCR - YAY! If you click on the image you can see it much larger and you'll notice that we seem to have DNA from all of the second elutions from the DNA extraction and one from the first elution.

Because this looks promising, we're going to proceed with our PCR clean up and then run the second gel. We will report back later.

-M